Illumina mRNA Library Preparation Workshop, June 14 - 15 Edinburgh Genomics and Illumina are excited to bring you a new lab-based workshop designed for genomicists looking to make their own high-quality NGS libraries ready for sequencing. This workshop will consist of both in-person presentations and hands-on lab work taking you through the theory and techniques of mRNA library preparation. Illumina, the forerunners of innovation in next-generation sequencing, will bring their unrivalled expertise to guide participants in the use of their own mRNA prep kits, whilst Edinburgh Genomics will provide a fully equipped genomics laboratory along with expert technicians to help with protocols. Instructors Cathlene Eland – Illumina Laura Ingram - Illumina Edinburgh Genomics lab team Workshop format The workshop consists of presentations and hands-on tutorials. Who should attend This course is primarily aimed at University of Edinburgh researchers and technicians who have used or plan to use Edinburgh Genomics sequencing facilities and plan to make their own libraries for sequencing in the future. It is suitable for postgraduates, technicians, and PIs, using NGS data in their research and who have some experience working in a molecular wet lab prior to the start of the course. Covered topics Day 1 Introduction to Illumina sequencing and review of Illumina stranded mRNA workflow Purification and fragmentaton of mRNA Synthesis of stranded cDNA Adenylation of 3' ends Ligation of anchors Fragment clean up Day 2 Amplification and clean up of Libraries Review and discussion Sequencing lab tour Pooling and sequencing of prepared libraries (EdGe) Related Links Edinburgh Genomics Jun 14 2022 10.30 - Jun 15 2022 17.00 Illumina mRNA Library Preparation Workshop, June 14 - 15 This workshop will consist of both in-person presentations and hands-on lab work taking you through the theory and techniques of mRNA library preparation. Register here
Illumina mRNA Library Preparation Workshop, June 14 - 15 Edinburgh Genomics and Illumina are excited to bring you a new lab-based workshop designed for genomicists looking to make their own high-quality NGS libraries ready for sequencing. This workshop will consist of both in-person presentations and hands-on lab work taking you through the theory and techniques of mRNA library preparation. Illumina, the forerunners of innovation in next-generation sequencing, will bring their unrivalled expertise to guide participants in the use of their own mRNA prep kits, whilst Edinburgh Genomics will provide a fully equipped genomics laboratory along with expert technicians to help with protocols. Instructors Cathlene Eland – Illumina Laura Ingram - Illumina Edinburgh Genomics lab team Workshop format The workshop consists of presentations and hands-on tutorials. Who should attend This course is primarily aimed at University of Edinburgh researchers and technicians who have used or plan to use Edinburgh Genomics sequencing facilities and plan to make their own libraries for sequencing in the future. It is suitable for postgraduates, technicians, and PIs, using NGS data in their research and who have some experience working in a molecular wet lab prior to the start of the course. Covered topics Day 1 Introduction to Illumina sequencing and review of Illumina stranded mRNA workflow Purification and fragmentaton of mRNA Synthesis of stranded cDNA Adenylation of 3' ends Ligation of anchors Fragment clean up Day 2 Amplification and clean up of Libraries Review and discussion Sequencing lab tour Pooling and sequencing of prepared libraries (EdGe) Related Links Edinburgh Genomics Jun 14 2022 10.30 - Jun 15 2022 17.00 Illumina mRNA Library Preparation Workshop, June 14 - 15 This workshop will consist of both in-person presentations and hands-on lab work taking you through the theory and techniques of mRNA library preparation. Register here
Jun 14 2022 10.30 - Jun 15 2022 17.00 Illumina mRNA Library Preparation Workshop, June 14 - 15 This workshop will consist of both in-person presentations and hands-on lab work taking you through the theory and techniques of mRNA library preparation.
