Genome assembly using Oxford Nanopore Sequencing course, April 11 -14 Overview Long-read genome sequencing technologies offer the opportunity to assemble highly contiguous genome sequences for a huge spectrum of organisms, including telomere-to-telomere human chromosomes, fully closed bacterial chromosomes and plasmids, and even entire viral genomes in single reads. A wide variety of tools exist to process long-read sequencing data, from basecalling through to genome assembly and polishing. Each of these has been developed to optimise the accuracy of the resulting genome assemblies, either in hybrid with paired short-read data, or using long reads alone. This course will introduce participants to a range of methods to complete the steps required to process raw Oxford Nanopore Technologies sequencing data into a fully assembled, polished and quality-controlled genome assembly, both with and without accompanying short reads, and with and without a reference genome. Over four days, we will include a combination of both theoretical background and practical application using model viral and bacterial datasets, concluding with a full run-through of the assembly, polishing and quality control pipeline at each course participants’ own pace. Target audience and assumed background This course is intended for researchers interested in learning the background and practical techniques involved in genome assembly using Oxford Nanopore Technologies data. Both beginners and more advanced users are welcome. Some background in navigating the command line would be useful, but we will cover the needed essentials throughout the hands-on sessions. Learning outcomes ● Learn the advantages and disadvantages of long-read sequencing ● Understand the steps involved in genome assembly using long read data ● Gain practical experience in choosing and using the optimal tools for a variety of dataset types, including microbiome, bacterial, viral and mammalian Instructors Dr. Amanda Warr, The Roslin Institute Dr. Natalie Ring, The Roslin Institute More info Physalia Courses Apr 11 2022 01.00 - Apr 15 2022 00.59 Genome assembly using Oxford Nanopore Sequencing course, April 11 -14 This course is intended for researchers interested in learning the background and practical techniques involved in genome assembly using Oxford Nanopore Technologies data. Register here
Genome assembly using Oxford Nanopore Sequencing course, April 11 -14 Overview Long-read genome sequencing technologies offer the opportunity to assemble highly contiguous genome sequences for a huge spectrum of organisms, including telomere-to-telomere human chromosomes, fully closed bacterial chromosomes and plasmids, and even entire viral genomes in single reads. A wide variety of tools exist to process long-read sequencing data, from basecalling through to genome assembly and polishing. Each of these has been developed to optimise the accuracy of the resulting genome assemblies, either in hybrid with paired short-read data, or using long reads alone. This course will introduce participants to a range of methods to complete the steps required to process raw Oxford Nanopore Technologies sequencing data into a fully assembled, polished and quality-controlled genome assembly, both with and without accompanying short reads, and with and without a reference genome. Over four days, we will include a combination of both theoretical background and practical application using model viral and bacterial datasets, concluding with a full run-through of the assembly, polishing and quality control pipeline at each course participants’ own pace. Target audience and assumed background This course is intended for researchers interested in learning the background and practical techniques involved in genome assembly using Oxford Nanopore Technologies data. Both beginners and more advanced users are welcome. Some background in navigating the command line would be useful, but we will cover the needed essentials throughout the hands-on sessions. Learning outcomes ● Learn the advantages and disadvantages of long-read sequencing ● Understand the steps involved in genome assembly using long read data ● Gain practical experience in choosing and using the optimal tools for a variety of dataset types, including microbiome, bacterial, viral and mammalian Instructors Dr. Amanda Warr, The Roslin Institute Dr. Natalie Ring, The Roslin Institute More info Physalia Courses Apr 11 2022 01.00 - Apr 15 2022 00.59 Genome assembly using Oxford Nanopore Sequencing course, April 11 -14 This course is intended for researchers interested in learning the background and practical techniques involved in genome assembly using Oxford Nanopore Technologies data. Register here
Apr 11 2022 01.00 - Apr 15 2022 00.59 Genome assembly using Oxford Nanopore Sequencing course, April 11 -14 This course is intended for researchers interested in learning the background and practical techniques involved in genome assembly using Oxford Nanopore Technologies data.
